Regenerated plants before their transfer to soil
revealed seedlings with morphological changes, including
shoots with deformed leaf, albino seedlings and rarely
chimerical plantlets with leaves in albino and green bands
(Fig. 1). The regeneration of albino plantlets was observed
in both genotypes Karim and Isly (Table I). No albino plants
were regenerated from a Sebou callus. On the other hand,
chimerical seedlings with foliar malformations were
observed in all three genotypes. After transfer to ground,
most F0 seedlings, monitored at maturity, showed a normal
phenotype. However, phenotypic variations were recorded
and involved mainly spike morphology and fertility (Fig. 2a,
b & c). Nevertheless, R1 plants, resulting from R0 that
present abnormality in spike morphology, did not show any
spike defect. The study of the variations observed in R1
somaclones (Fig. 2d) showed significant differences in most
studied characters
Regenerated plants before their transfer to soilrevealed seedlings with morphological changes, includingshoots with deformed leaf, albino seedlings and rarelychimerical plantlets with leaves in albino and green bands(Fig. 1). The regeneration of albino plantlets was observedin both genotypes Karim and Isly (Table I). No albino plantswere regenerated from a Sebou callus. On the other hand,chimerical seedlings with foliar malformations wereobserved in all three genotypes. After transfer to ground,most F0 seedlings, monitored at maturity, showed a normalphenotype. However, phenotypic variations were recordedand involved mainly spike morphology and fertility (Fig. 2a,b & c). Nevertheless, R1 plants, resulting from R0 thatpresent abnormality in spike morphology, did not show anyspike defect. The study of the variations observed in R1somaclones (Fig. 2d) showed significant differences in moststudied characters
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