The mercury speciation content in seafood and CRM samples was determined by LC-UV-CV-AFS following microwave extraction. The samples and CRMs were weighed in digestion vessels (0.1e1 g) and 10 mL of hydrochloric acid 4 mol L1 were added to perform a microwave-assisted extraction (MAE). The microwave extraction procedure was as follows: 2 min from room temperature to 100 C and 10 min maintained at 100 C. After cooling to room temperature, the extraction samples were filtered through paper filters