Total antioxidant activities of extracts were determined according
to the method described by Rai et al. (2011). Briefly,
0.2 mL of extract was mixed with 3.0 mL reagent solution
mixture (0.6 M sulphuric acid: 28 mM sodium phosphate: 4mM
ammonium molybdate – 1:1:1). The reaction mixture was
incubated at 95 °C for 90 min in a water bath. After incubation
the contents were cooled to room temperature and
Soybean
Washed and soaked overnight (16 h)
Drained extract
Soaked soybean
Cooked soybean
Add water
Soybean: water – 1:1
Pressure cooked
dehulling
Dehulled seed
Solid state fermentation*
Cooked extract
Fermented soybean * Inoculum – 5 % fermented soybean
Starter – B. subtilis MTCC1747, B. subtilis
MTCC5480
Time – 24 h
Temperature – 42±1⁰C
Seed coat
Fig. 1 – Schematic representation of processing and
fermentation of soybean.