The essential oil was analysis by using a Perkin Elmer Clarus
600 GC System, fitted with a Rtx-5MS capillary column
(30 m 0.25 mm i.d. 0.25 mm film thickness; maximum
temperature, 350 C), coupled to a Perkin Elmer Clarus 600C
MS. Helium gas with purity 99.9999% was used as carrier gas at
a constant flow of 1.0 ml/min. The injector, transfer line and
ion source temperatures were 280, 270 and 270 C, respectively.
The ionizing energy of MS was 70 eV. Electron multiplier
(EM) voltage was obtained from autotune. All data were
obtained by collecting the full-scan mass spectra within the
scan range 40e550 amu. The injected sample volume was
0.5 ml with a split ratio of 200:1. The oven temperature program
was 50 C at a rate of 3 C/mine280 C hold for 10 min. The
unknown compounds were identified by comparing the
spectra obtained with mass spectrum libraries (NIST 2011
v.2.3 and Wiley, 9th edition).