Fresh leaves of lemongrass (C. citratus) were collected at the Medicinal Plant Nursery of the Federal University of Lavras located in Lavras, Minas Gerais, Brazil
(21°14’43” S; 44°59’59” W; altitude 919 m). The harvest
was carried out in January 2009, in the morning, with mild
temperatures and absence of rain period. Dry clove flower
buds (S. aromaticum) were acquired in a retail location. For
confirmation, both vegetal species were submitted a characterization procedure at botany sector of Federal University of Lavras. The EO was extracted by hydrodistillation
using a modified Clevenger apparatus. Plant material was
placed with water in a 6000 mL volumetric distillation
flask. The flask was coupled to the modified Clevenger apparatus, and the extraction was performed for 3 h with the
temperature maintained at 100 5 °C. The obtained hydrolate (water/oil fraction) was centrifuged at 321.8 g for
10 min at 25 °C. The EO was collected with a Pasteur pipette, and the water traces were removed with anhydrous
sodium sulfate (Vetec®
, Brazil). The oil was stored under
refrigeration temperature (52 °C) in glass flasks wrapped
in aluminum foil (Guimarães et al., 2008)
Fresh leaves of lemongrass (C. citratus) were collected at the Medicinal Plant Nursery of the Federal University of Lavras located in Lavras, Minas Gerais, Brazil
(21°14’43” S; 44°59’59” W; altitude 919 m). The harvest
was carried out in January 2009, in the morning, with mild
temperatures and absence of rain period. Dry clove flower
buds (S. aromaticum) were acquired in a retail location. For
confirmation, both vegetal species were submitted a characterization procedure at botany sector of Federal University of Lavras. The EO was extracted by hydrodistillation
using a modified Clevenger apparatus. Plant material was
placed with water in a 6000 mL volumetric distillation
flask. The flask was coupled to the modified Clevenger apparatus, and the extraction was performed for 3 h with the
temperature maintained at 100 5 °C. The obtained hydrolate (water/oil fraction) was centrifuged at 321.8 g for
10 min at 25 °C. The EO was collected with a Pasteur pipette, and the water traces were removed with anhydrous
sodium sulfate (Vetec®
, Brazil). The oil was stored under
refrigeration temperature (52 °C) in glass flasks wrapped
in aluminum foil (Guimarães et al., 2008)
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