Subsequently, the
hydrolyzed glucose solution was adjusted to pH
5.5 by the addition of 20 % Ca(OH)2 solution.
Then, this solution was autoclaved at 121°C for
20 min. The ethanol fermentation process was
performed in a total volume of 125 mL using 5
g/L S. cerevisiae cells, 50 g/L glucose, 0.5 g/L
(NH4)HPO4, 0.025 g/L MgSO4.7H2O, 1.2 g/L
NaH2PO4 and 1 g/L yeast extract at 30°C for 72