Biosynthesized silver nanoparticles were synthesized with the aid of a novel, non-toxic, eco-friendly biological
material, namely aloe leaf extract (ALE). Crushed, ultrasound treated, decolorized aqueous aloe
leaf extract was used for capping silver nitrate. Silver nanoparticles were formed when the reaction conditions
were altered with respect to concentration of silver nitrate, consumption of hydrazine hydrate,
ALE content and incubation temperature. The colorless reaction mixtures turned brown and displayed
UV–visible spectra characteristic of silver nanoparticles. Transmission electron microscope (TEM) observation
revealed the predominance of silver nanosized crystallites after short incubation period. After the
reaction mixtures were incubated for 30 days, the presence of silver nanoparticles was confirmed by
X-ray diffraction analysis. Great antimicrobial activity against bacterial cultures was displayed by these
formed silver nanoparticles.