The filtrate was evaporated in a water bath at 40 C to a final volume 1 ml. Antioxidant activity was determined by measuring the coupled oxidation of carotene and linoleic acid, as described by Hammerschmidt and Pratt (1978).
The filtrate was evaporated in a waterbath at 40 C to a final volume 1 ml. Antioxidant activitywas determined by measuring the coupled oxidationof carotene and linoleic acid, as described by Hammerschmidtand Pratt (1978).