Foremost, the ability of the system to work in enclosed fluidic media was proven. For this purpose, we have
fabricated a prototype based on (i) a mechanical holder which allows the positioning of the silicon device, (ii) a
PDMS homemade gasket adapted to the cell dimension and (iii) an aluminum cover to insure homogeneous pressure
on the chip which allow a perfect sealing of the system, see Fig. 3 (3).
The prototype is then connected, by Teflon capillaries having an internal diameter of 0.5 mm, with a system
consisting of a syringe pump and an injection loop of biological samples. In order to test the fluidic circuit (cell and
capillaries) fluids at different flow rates were injected, the experiments showed that injection of 10 seconds at a flow
of 3 mL/min permit to fill the fluidic circuit without trapping air bubbles.