Algal strain and wastewater samples
A pure strain of Chlorella minutissima was
procured from Centre for Blue Green Algae, Indian
Agricultural Research Institute (IARI), New Delhi,
India. Algal strain was grown in a complex BG-11
medium9 on tissue culture rack (Vista Biocell Pvt.
Ltd., India) adjusted at pH of 7.0 under an
illumination 6500 ± 300 lux with regime 16:8 h (light:
dark) at 27 °C. Sewage wastewater was taken from
the drain of local hotel. After collection wastewater
samples were filtered from Whatman filter paper
No. 1 in order to remove large suspended solid
particles and then exposed in UV light for
sterilization.