the whole fish was dried in an electric oven at 70 – 80 for three days. the fish samples were pulverized using clean mortal and pistil to produce it powdered form.
A homogenized 2 g of the grounded fish was weighed in analytical balance and ashed in a furnace at 550 c. The samples were put in flask and 10 ml each of concentrated were added. The samples were digested for 2-3 hours,filtered into 100 cm standard volumetric flask and made to mark with distilled water