PVDF membrane is an ideal support for amino-terminal sequencing, amino acid analysis and immunoassays of blottied proteins. PVDF retains protein under extreme conditions of exposure to acidic or basic conditions, and in the presence of organic solvents. Greater retention during sequencing manipulation enhances the likelihood of obtaining information from rare, low abundance protein, by increased initial coupling and higher repetitive yields.
In addition, PVDF membrane exhibits better binding efficiency of blotted material in the presence of SDS in the transfer buffer.