b s t r a c t
Citrus aurantium L. (bitter orange) is frequently used as an ingredient of food supplements aimed to
reduce body weight or improve general physical performances. The most active compounds of
C. aurantium are amines having adrenergic activity: octopamine, synephrine, tyramine, N-methyl-tyramine
and hordenine. The quantification of these amines is critical since their content in food supplements
is regulated by national/international rules. Some methods for the quantification of C. aurantium
amines have been published, including the official method developed by AOAC, but most of them are not
totally satisfactory for the analysis of complex matrixes, such as extracts or food supplements.
A new HPLCeUVefluorescence procedure has therefore been developed; the method is quick and
simple, and allows the analysis of samples after a rapid extraction procedure without any further
cleaning step. The assay, using one or two detectors, showed good results during the validation tests
performed according to the FDA guidelines.
b s t r a c t
Citrus aurantium L. (bitter orange) is frequently used as an ingredient of food supplements aimed to
reduce body weight or improve general physical performances. The most active compounds of
C. aurantium are amines having adrenergic activity: octopamine, synephrine, tyramine, N-methyl-tyramine
and hordenine. The quantification of these amines is critical since their content in food supplements
is regulated by national/international rules. Some methods for the quantification of C. aurantium
amines have been published, including the official method developed by AOAC, but most of them are not
totally satisfactory for the analysis of complex matrixes, such as extracts or food supplements.
A new HPLCeUVefluorescence procedure has therefore been developed; the method is quick and
simple, and allows the analysis of samples after a rapid extraction procedure without any further
cleaning step. The assay, using one or two detectors, showed good results during the validation tests
performed according to the FDA guidelines.
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