Fresh pomegranate fruits (P. granatum L. cv. Rabbab-e Neyriz) were
obtained from a local super market. The fruits were washed and cut into
four pieces. The seeds/arils were manually separated and ground in a
mixer for 30 s and then passed through muslin cloth. After filtering by
a Millipore filter with a 0.22 μm nylon membrane under vacuum at
25 °C, the freshly prepared juice was used for treatments.