Lee et al. (2002) centrifuged the sea water contaminated
with Vibrio sp and washed with sterilized distilled water to prepare
salt-free cell suspension. They performed the multiplex PCR to
explore the field applicability of the species-specific primer, and
observed that all the target genes were detected. Their primers
were detectable for V. vulnificus from octopus infected with the strain.
However, in this study, even the DNA extracted from the samples
collected from the nature, showed no detection from octopus. It might
be due to negligible numbers of the strain in samples.