Data were analyzed using Rotor Gene -
software, version 2.0.2 (build 4) (Qiagen, Hilden, Germany) and LinRegPCR
software version 2012.0 (Amsterdam, Netherland), to give the threshold cycle
number and reaction efficiency (Ruijter et al., 2009). Relative transcript levels were
calculated using efficiency adjusted Pfaffl methodology (Dorak, 2006).