Galactosidase ( D-galactoside galactohydrolase, EC. 3.2.1.22), is an important enzyme in the processing of galactooligosaccharides that hydrolyzes galactooligosaccharides especially raffinose and stachyose found in most of the leguminous foods, as these sugar are responsible for intestinal discomfort and flatulence. -galactosidase producing bacteria are isolated. Optimization of parameters like pH, temperature and agitation speed for the fermentation process found 7 8, 36 38oC and 170 rpm respectively. In the nutritional parameter optimization raffinose found to be effective carbon source from many bacterial isolates for alpha-galactosidase production. Peptone preferred to be best source of nitrogen for fermentation medium of galactosidase after NM 4 NO 3 for isolated bacteria. Effect of K2HPO4 and MgSO4.7H 2 O. as a source of mineral found to enhancer of enzyme production in the medium.