X-ray emission generated by ion beam bombardment of target
materials could be one of the potential factors to cause DNA change
and thus genetic mutation of biological cells. However, this effect
significantly depended on the material used to make the sample
holder. Metal holders could emit X-ray induced by ion beam bombardment and thus enhanced the mutation frequency. But, ionbeam-generated X-rays from both the holder made from light-elements composed materials such as polymers and the biological living samples were undetectable and thus negligible, and therefore
the secondary effect from these X-rays on the mutation induction
was negligible. Therefore, the answer to the question of this paper
title is that the X-ray emission induced by low-energy ion beam
from the ion-bombarded biological sample itself is not a secondary
mutation source.