DPPH radical scavenging activity of the extracts was determined
according to the method described by Rai et al. (2011).
Briefly, 2.0 mL of 0.16 mM of DPPH solution was added to 200 L
aliquot of extracts.The content was vortexed for 1 min and incubated
at room temperature for 30 min in the dark. After
incubation, the absorbance of all samples was measured at
517 nm.The scavenging effect was measured according to the
formula: