Crystallization is a widely used separation process in a variety of
industrial applications. Fortunately, the culture broth of the C.
militaris mutant G81-3 obtained by the high-energy proton beam
irradiation in our previous study, has high cordycepin
concentration (12,13). Therefore, one step recovery of cordycepin
from this culture broth by crystallization might be a promising
method to produce purified cordycepin in bulk. In this study, some
process factors for cordycepin crystallization from the culture broth
of G81-3 were investigated to obtain a highly purified product with
a high yield.