Microbial analysis
Thirty grams of cucumber fruit pulps was
removed aseptically from each treatment. The
sample was then homogenized in peptone
saline solution (8.5 g/l NaCl + 1 g/l peptone for
1 min in a stomacher (S400, Shanghai
Scientific Instrument Co., Ltd., Shanghai,
China). After making serial dilutions in peptone
water, the samples were plated on different
media as follows: (1) plate count agar (PCA),
for isolating total aerobic psychrotrophic
micro-organisms was incubated at 12oC for 72
h and mesophilic micro-organisms was
incubated at 30oC for 72 h; (2) Sabouraud
media (Oxoid CM41) for isolating yeasts and
moulds was incubated at 25oC for 120 h.
Colonies were counted and the results
expressed as CFUg-1 of cucumber. Analyses
were carried out periodically in randomly
sampled from the baskets. Two replicate counts
were performed for each tray (ICMSF, 1983).
Microbial analysis
Thirty grams of cucumber fruit pulps was
removed aseptically from each treatment. The
sample was then homogenized in peptone
saline solution (8.5 g/l NaCl + 1 g/l peptone for
1 min in a stomacher (S400, Shanghai
Scientific Instrument Co., Ltd., Shanghai,
China). After making serial dilutions in peptone
water, the samples were plated on different
media as follows: (1) plate count agar (PCA),
for isolating total aerobic psychrotrophic
micro-organisms was incubated at 12oC for 72
h and mesophilic micro-organisms was
incubated at 30oC for 72 h; (2) Sabouraud
media (Oxoid CM41) for isolating yeasts and
moulds was incubated at 25oC for 120 h.
Colonies were counted and the results
expressed as CFUg-1 of cucumber. Analyses
were carried out periodically in randomly
sampled from the baskets. Two replicate counts
were performed for each tray (ICMSF, 1983).
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