Shrimp waste is an important source of bioactive molecules and it undergoes rapid disintegration which leading to
environmental pollution. It is necessary to preserve the material adopting the environmentally safe techniques, prior to
recovery of bioactive components such as proteins and carotenoids. Aqueous two-phase system (ATPS) partitioning has been
used to recover and concentrate proteins from Shrimp Waste and offers many advantages along with biomass removal.
Hence, a feasible protocol for the recovery of protein from Shrimp Waste has been established. The best conditions of
partitioning were achieved using ATPS composed of PEG 4000, ammonium citrate salt, pH8, 1M addition of sodium
chloride, and 36.28 Tie line length. The maximum percentage yield of protein extracted from shrimp waste was found to be
74.50%.