Figure 5. L. rhamnosus up-regulates and redistributes p21 in ME-180 cells. Lactobacilli were allowed to colonize a non-confluent layer of ME-
180 cells for 24 hours. The cells were prepared for qPCR analysis or immunofluorescence. The graph shows the normalized relative p21 mRNA levels
in ME-180 cells upon colonization by the three different lactobacillus strains. Three independent experiments were analyzed (*indicates that p,0.05)
(A). Immunofluorescence images displaying the p21 protein localization and expression for both ME-180 cells alone and ME-180 cells that have been
colonized with L. rhamnosus for 24 hours. The cellular DNA was also stained with DAPI. The scale bar represents 10 mm