The efficacy of 0.02% vitamin C (VC;L-ascorbic acid) and 0.05% b-carotene (BC) at the rate of 1 ml/100 g of body weight in
amelioration of ethyl methane sulphonate (EMS)-induced genotoxicity has been studied in an Indian endemic fish,Anabas testudineusby
using several cytogenetical endpoints like chromosome aberrations, micronuclei (MN) and abnormal nuclei (AN), and sperm head
anomaly at 6, 24, 48, 72 and 96 h after treatment, as compared to suitable controls (distilled water (DW)-treated control for EMS and
VC-treated fish, and 1% alcohol-treated control for BC-treated fish). Both VC and BC reduced EMS-induced genotoxicity at all the
fixation intervals as compared to their respective controls. Additionally, effects of two more doses of VC (0.01% and 0.05%) and BC
(0.02% and 0.1%) were analyzed at 72 h after treatment (at the peak period of EMS genotoxicity) for testing their relative efficacy in
amelioration of EMS-induced cytogenetical damage in this fish. All the three doses of both VC and BC appeared to reduce the EMSinduced genotoxicity in this fish to a variable extent, of which the higher dose of VC appeared to give marginally better protection while
the dose–response relationship was inconclusive for BC. The results of this study can lead to future research for exploring if low doses of
these vitamins may be useful in protecting fish from genotoxic damage on exposure to mutagenic agents in small confined/stagnant
waters.
The efficacy of 0.02% vitamin C (VC;L-ascorbic acid) and 0.05% b-carotene (BC) at the rate of 1 ml/100 g of body weight inamelioration of ethyl methane sulphonate (EMS)-induced genotoxicity has been studied in an Indian endemic fish,Anabas testudineusbyusing several cytogenetical endpoints like chromosome aberrations, micronuclei (MN) and abnormal nuclei (AN), and sperm headanomaly at 6, 24, 48, 72 and 96 h after treatment, as compared to suitable controls (distilled water (DW)-treated control for EMS andVC-treated fish, and 1% alcohol-treated control for BC-treated fish). Both VC and BC reduced EMS-induced genotoxicity at all thefixation intervals as compared to their respective controls. Additionally, effects of two more doses of VC (0.01% and 0.05%) and BC(0.02% and 0.1%) were analyzed at 72 h after treatment (at the peak period of EMS genotoxicity) for testing their relative efficacy inamelioration of EMS-induced cytogenetical damage in this fish. All the three doses of both VC and BC appeared to reduce the EMSinduced genotoxicity in this fish to a variable extent, of which the higher dose of VC appeared to give marginally better protection whilethe dose–response relationship was inconclusive for BC. The results of this study can lead to future research for exploring if low doses ofthese vitamins may be useful in protecting fish from genotoxic damage on exposure to mutagenic agents in small confined/stagnantwaters.
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