2. Materials and methods Vali-e-Asr hospital in Khorramshahr city was noticed in this research. This hospital was among the largest public health centers in the city. The samples were gathered by direct collection (sterile hand gloves and containers) from different parts of hospital, laboratory and kitchen. The hunted samples were transferred to a lab in sterile tubes. Totally 20 cockroaches were identified according to the standard and reliable systematic keys. Just whole and alive cockroaches were examined in this study. Cockroaches were immobilized at 4 °C for 10-20 min. Any cockroach’s body washed fully by 5 cc physiological sterile serum. Different selective bacterial media such as eosin methylene blue agar, blood agar and salmonella shigella agar were applied for culturing bacteria. Bacteriological recognition tests were performed by an expert bacteriologist.