3.4. Reproducibility
The overall reproducibility of the multi-allele genotyping assay
(including extension reaction and the dipstick test) was assessed by
analysing nine times (three PEXT reaction products analysed in triplicate)
PCR products from genomic DNA obtained from three individuals
of different genotype (wild type, herozygous and homozygous mutant
for Hb Icaria). According to the results (Fig. 3B) the reproducibility of
the allele's detection in each sample is very satisfactory.