Fatty acid analysis was performed in a Shimadzu GC 2010 gas chromatograph equipped with a FID detector, under the following conditions: split injection (1:30 injection volume 1 lL); capillary column SP 2560 (Supelco) (100 m 0.25 lm 0.2 lm film); injection port and detector temperatures of 250 C and 260 C respectively; initial oven temperature of 140 C, increasing at 4 C/min to 240 C, which was maintained for 15 min; and carrier gas of hydrogen at 1.5 mL/min flow rate