Methodaccuracyresultsobtainedbycalculatingtherelativestandarddeviation(RSD)valuefortheretentiontime(Rt)andaveragepeakarearatio(Pa)ofalkaloidstointernal standard from six replicate injectionswithin intra and inter-day runs in a mixture of alkaloids and IS (caffeine) all at a concentration of 5g/mL.