JA stem is a quality feedstock for bioethanol production due to
rich soluble sugar and low ash. Correlation analysis of 59 diverse JA
accessions has indicated that both sugar and ash significantly
affect biomass saccharification. Comparative analyses of four
alkali-based pretreatments indicate that pretreatment D is the
most suitable method for biomass digestion. Notably, Lignin and
pectin removal could distinctively affect the cellulose CrI and DP
for high biomass saccharification. Furthermore, H2O2, contrary to
ultrasound, displays a great effect on hydrolysate detoxification
by largely reducing the generation of acetic acid during alkalibased
pretreatments.