Mushrooms were harvested when the mushroom cap surface were flat to slightly up-rolled at the cap margins. The harvested fruiting bodies in each bag were then counted and weighed. At the end of the harvest period, the accumulated data were used to calculate the biological efficiency and mushroom weight.
Biological efficiency (%) = eight of fresh mushrooms harvested per bag/weight of dry substrate per bag × 100.
Mushroom weight (g) = Total weight of harvested fresh mushrooms per bag/total number of mushrooms harvested per bag.