2.9. Sensitivity testing of multiplex PCR and monoplex PCR To compare the sensitivity level of multiplex PCR and monoplex
PCR, 10-fold serial dilutions (10−1 to 10−4) in distilled water of thefour different viral cDNA of Aichi virus, HPeV, enteroviruses, andHBoV were tested by multiplex PCR and monoplex PCR. The multiplex PCR and monoplex PCR were carried out simultaneously for the same dilution series with the same PCR machine.