2.2 Preparation of wheat and chickpea meal
Wheat (Triticum aestivum L. cv AC Vista) grown in a field at Saskatoon (52° 8′ N/106°
38′ W and 481.5 m altitude) and chickpea (Cicer arientinum L. RIL 00047) in a greenhouse
(temperature minimum 18 and maximum 23 °C with an 18 h photoperiod and 385 μM m−2 s −1 of
photosynthetically active radiation) grains were used for mineral concentration analysis. Five
grams of grains were ground and passed through a 0.5 mm sieve in UDY mill (UDY
Corporation, Fort Collins, CO, USA). The ground grain material was used immediately or stored
in a desiccator. The moisture content of the seed meal samples was determined using AACCI
approved method 44-01.01 [27].