Reconstituted milk was prepared using 12% non-fat milk powder (Enka, Konya, Turkey); then it was heat treated at 85 °C for 15 min. Reconstitued milk was inoculated with natural Kefir grains (2%) and incubated at 25 °C for 20 h. The Kefir grains were separated by filtration, and the fermentate was used as a natural starter culture in kefir production. Milk samples were inoculated with the natural starter culture and incubated at 25 °C for approximately 20 h until pH 4.6 was reached. Kefir samples were stored at 4 °C for a day. The information regarding sample design is shown in Table 1. Both Kefir samples and milk samples were analyzed.