A new set of SSRs specific to marigold was developed
using the ISSR technique and SSR-enriched genomic
DNA library. Through the ISSR technique, 32 SSRs were
developed, and the results indicated that 20 SSRs could
amplify specific products. Of these, 11 SSRs exhibited
polymorphism within the studied population and
accounted for 34.38% of the developed SSRs. Among
the 98 SSRs derived from the SSR-enriched genomic
DNA library, 41 SSRs were specifically amplified and 25
SSRs (25.51% of the total developed SSRs based on a
SSR-enriched genomic DNA library) were polymorphic
markers. Primer sequences and accession numbers are
given in Table S1 (available online). In addition, investigation
of the transferability of SSRs originating from sunflower
revealed that 35 of the 63 pairs of SSR primers
could amplify the DNA of marigolds, but only two pairs
(3.17%) exhibited polymorphism as indicated in Fig. 1
(an example of an amplified DNA by SSR is shown in
Fig. S1, available online).