Serial dilutions (1/10) were performed with 0.1 g/100 g peptoneewater(Laboratorio Britania, Buenos Aires, Argentina). 1 mL from appropriate dilutionwas put inpetri dish and puton 5 mL dilution of agar medium (Laboratorio Britania, Buenos Aires, Argentina) for total count. Petri dishes were incubated for 48 h at 35?C. The result was expressed as colony forming units (CFU)