Even though many automatic analysers have been developed to reduce the dead volume of reagents in cassettes, approximately 1– 3 mL of reagent remains in a reagent cassette because the reagent probe of automated machines does not extend into the enzyme reagent deeper than its sensor level. Generally, the remaining reagents that are left in the cassettes are neglected in clinical laboratories. They are discarded daily, even though the reagents still contain active ingredients, i.e., enzymes and substrates. These small reagent volumes can be utilised in miniaturised systems that consume low reagent volumes, such as microfluidic devices or paper-based analytical devices (PADs). The latter approach is promising for being developed as a point-of-care-testing (POCT) for use in developing countries [29] because of its simplicity and an extremely low-cost of paper substrates.
Even though many automatic analysers have been developed to reduce the dead volume of reagents in cassettes, approximately 1– 3 mL of reagent remains in a reagent cassette because the reagent probe of automated machines does not extend into the enzyme reagent deeper than its sensor level. Generally, the remaining reagents that are left in the cassettes are neglected in clinical laboratories. They are discarded daily, even though the reagents still contain active ingredients, i.e., enzymes and substrates. These small reagent volumes can be utilised in miniaturised systems that consume low reagent volumes, such as microfluidic devices or paper-based analytical devices (PADs). The latter approach is promising for being developed as a point-of-care-testing (POCT) for use in developing countries [29] because of its simplicity and an extremely low-cost of paper substrates.
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