Cassava is one of the most efficient and rich crops in terms of carbohydrate production, which is a tropical
perennial plant that grows on poor or depleted soils. Microbial conversion of such a renewable raw material
to useful products is an important objective in industrial biotechnology. L-Lactic acid was efficiently
produced from cassava powder by a Lactobacillus rhamnosus strain CASL. The fermentation properties of
cassava powder were compared with those of glucose and corn powder. The efficiencies of various
fermentation strategies for L-lactic acid production from cassava powder, including simultaneous saccharification
and fermentation (SSF), two-step fermentation (TSF) and simultaneous liquefaction,
saccharification and fermentation (SLSF), were investigated. The high L-lactic acid concentration
(175.4 g/l) was obtained using 275 g/l of cassava powder concentration (total sugar of 222.5 g/l) in SSF
batch fermentation. This is the highest L-lactic acid concentration reported, from cassava source, and it provides
an efficient L-lactic acid production process with cheap raw bioresources, such as cassava powder.