Positive interactions between Pichia anomala and S. cerevisiae have been described for the ester profile of the wine (no excess of ethyl acetate, increase in isoamyl acetate) [39]. Mixed fermentation of Pichia kluyveri and S. cerevisiae enhanced the volatile thyols in comparison with pure cultures. More recently, the comparison between monocultures and co-cultures revealed yeast interactions for the aroma profile of a Savignon Blanc wine. A synergistic effect on the aroma profile of the wine was seen for mixed fermentation with M. pulcherrima and S. cerevisiae, while C. zemplinina and S. cerevisiae co-cultures showed negative interactions, with a decrease in the terpene and lactone contents [15•]. Another synergistic effect was shown in mixed fermentation using L. thermotolerans and S. cerevisiae, where an increase in glycerol and 2-phenyl ethanol production in comparison with pure cultures was shown [12]. Future perspectives in this specific research topic should take into account of two aspects: i) the composition of the grape juice; ii) the scale-up of the fermentation trials. Indeed, the analytical profile of the wines from mixed fermentation may differs depending on the substrate and the fermentation scale. In our opinion further studies in this direction may contribute to a better understanding of the microbial interactions as well as the positive influence of mixed fermentation on the analytical profile of wines.