The acaricide-susceptible two-spotted spidermitewas obtained from
Gyeongnam Agricultural Research and Extension Services in 2007. The
colony was maintained at 25 ± 2 °C, 60 ± 10% relative humidity, and
16:8 h light:dark in the laboratory on kidney bean (Phaseolus vulgaris
var. humilis) plants without any exposure to any pesticides until they
were used in experiments. The fresh un-infested kidney bean plants
bearing three or four leaves were placed between the bean plants
infested with two-spotted spider mites for 24 to 48 h. During this time,
adults would move on to the un-infested plants, and the mites moved
to fresh bean leaves were used in subsequent experiments