Peclyve LI, the preparation yielding the greatest improvement in lycopene extraction, was used to investigate the effects of solvent type and enzyme incubation time on yields. Hexane, ethyl acetate or the ternary mixture hexane/acetone/ethanol (50:25:25 v/v) were used as solvent. The latter was chosen because of its proven efficacy for the extraction of carotenoids from plant material (Olives Barba et al., 2006). The enzyme incubation time was varied between 1 and 15 h. Lycopene extraction was carried out according to the procedure described in the previous section. The temperature and the extraction time were set at 40 °C and 3 h, respectively. These values were found to be a good compromise between the efficiency of extraction and the loss of lycopene due to oxidation.