As shown in Fig. 1, anthocyanin eluents were separated under
the experimental conditions. Twelve major peaks, possessed typical
anthocyanin spectra of a maximum absorbance at around
520 nm, were separated in addition to internal standard, cyanidin-
3,5-diglucoside. Peaks 8, 9 and 10 were the major anthocyanins
and they contributed to near half of the total anthocyanin
peak areas. Peak number, retention time, and% of total peak areas
were summarised in Table 1.