Using the column method, CR11 had superior Cd removal efficacy to that of CR20. Thus, we evaluated chemical components and functionality of fish sauce after treatment with CR11-packed columns (Table 1). Total nitrogen and free amino acid contents did not differ between untreated fish sauce and the first fraction(1.8 ± 0.04 vs. 1.8 ± 0.02 g/100 mL and 10,600 ± 200 vs.10,200 ± 300 mg/100 mL, respectively) and did not change in subsequent fractions. Thus, nutritional peptides and free amino acids remained in fish sauce samples after Cd removal using CR11.In subsequent metal ion analyses, Cu and Zn concentrations in untreated fish sauce were 1.4 ± 0.01 and 2.4 ± 0.04 mg/100 mL and were decreased to approximately 0.9 and 0.1 mg/100 mL