The work presented here compares the substrate specificity of the various groups of lipases and also looks at the enantioselectivity ofCaLBandsomeofitsreportedvariants,M72L,T103G,andW104H the native peptide signal were sub-cloned under the control of AOX1 promoter. The resulting constructs were used to transform Escherichia coli DH5. The plasmids were subjected to DNA sequencing for confirming the sequence