We have developed a fast, simple, and accurate DNA-based screening method to identify the fish species present in fresh and processed seafood samples.This versatile method employs PCR amplification of genomic DNA extracted from fish samples, followed by restriction fragment length polymorphism (RFLP) analysis to generate fragment patterns that can be resolved on theAgilent 2100 Bioanalyzer and matched to the correct species using RFLP pattern matching software.