Efficient protocols were established for in vitro seed germination, neo-formation of secondary (28)
protocorms from primary (18) protocorms and multiple shoot buds and protocorm-like body (PLB)
induction from pseudo-stem segments of in vitro-raised seedlings of Cymbidium giganteum. Four nutrient
media, namely Murashige and Skoog (MS)Phytamax (PM), Mitra et al. (M), and Knudson ‘C’ (KC) were
evaluated for seed germination and early protocorm development. In addition, the effects of peptone,
activated charcoal (AC) and two plant growth regulators [6-benzylaminopurine (BAP) and 2,4-
dichlorophenoxyacetic acid (2,4-D)] were also studied. Both M and PM supplemented with 2.0 g l1
peptone or 1.0 mg l1 BAP resulted in 100% seed germination. Media supplemented with 2.0 g l1 AC
could effectively induce large protocorms (1.6 0.1 mm in diameter). Ne