Effects of culture conditions on the growth and reproduction of Gut Weed, Ulva intestinalis Linnaeus (Ulvales, Chlorophyta).
In vitro cultivation of Gut Weed, Ulva intestinalis Linnaeus, was experimentally studied to support its near optimal farming, with potential impact in Thailand on its direct use as human food or its co-cultures in shrimp farming. Germling clusters (2 weeks old and 7.50±2.98 mm long) were seeded into 500 mL flasks and biomass growth rate optimized with respect to the main controllable factors; seedling density, salinity, light intensity, and temperature. These factors were assumed to each have an optimal value independent of the others, and the factors were optimized one at a time. The maximum growth at three to four weeks of cultivation was obtained at the factor levels of 0.05 gL-1, 20 ppt, 80 µmol photon m-2s-1 and 25°C. Early zoosporangia were obtained from 2nd to 4th weeks. The relative growth rate ranged from 9.47 to 22.18 % day-1, and only asexual reproduction of U. intestinalis was observed under these culture conditions. [ABSTRACT FROM AUTHOR]
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