The HaLACS 1 and HaLACS 2 were characterized in order to
define their role in the synthesis of TAGs in sunflower seeds. The
genes did not display any peptide domain that could be related
to their subcellular location, as public servers failed to predict
any for them. However, the ARAMENNOM database did predict
the presence of a hydrophobic domain probably involved in the
anchoring of the enzyme to cell membranes. The location of these
sunflower LACSs was studied by generating fluorescent-proteintagged versions of HaLACS 1 and HaLACS 2 and then transiently
expressing (via biolistic bombardment) these fusion proteins in
tobacco (N. tabacum) suspension-cultured cells, followed by