Conclusions
VC in commercial juices and vegetable crops was directly and fast determined by using an integrated biosensor based on the biocompatible conducting PEDOT-LS-AO composite film, which were fabricated by a co-electrodeposition technique. PEDOT-LS film could not only provide a biocompatible microenvironment for biologically-active AO molecules, but also effectively enhance the direct electron transfer between enzyme molecules and electrode interface. AO molecules immobilized in PEDOT-LS film exhibited excellent bioelectrocatalytic activity to the oxidation of VC. The designed biosensor exhibited good linear range, low working potential, fast current response, high sensitivity and bioaffinity. Moreover, the specificity, operational stability and repeatability of the proposed biosensor were also investigated with satisfactory results. Inspired by the preceding studies, the fabricated biosensor was employed for the determination of VC in commercial juices and vegetable crops. In addition, the further improvement of the fabricated biosensor for the determination and analysis of VC in fruits, vegetable and food crops, agricultural products and processed foods is also in progress.