The statistical optimization revealed an increase of phytase production by 5-fold by Bacillus subtilis US417 using wheat bran as a supporting material for solid state fermentation (Kammoun et al. 2012) 1.3-fold for Rhizomucor pusillus (Chadha et al. 2004), 1.8-fold for Mucor racemosus (Bogar et al., 2003a), 1.7-fold for Aspergillus ficuum (Bogar et al., 2003b), 1.75-fold for Pichia anomala in synthetic medium (Vohra and Satyanarayana 2002) and 5 fold for cane molasses medium (Kaur and Satyanarayana 2005). These observations clearly suggested that the nutritional and physical requirements of the microbes
differ from one another, and therefore, need to be optimized for each strain.