the proteins in the supernatant were precipitated by adding four fold volume of ice-cold acetone containing 10% TCA and 0.07% (w/v) β-ME, incubated at -20°C for at least 3 h, and then centrifuged for 15 min at 12 000 × g.
the proteins in the supernatantwere precipitated by adding four fold volumeof ice-cold acetone containing 10% TCA and 0.07% (w/v)β-ME, incubated at -20°C for at least 3 h, and thencentrifuged for 15 min at 12 000 × g.